Swiftly receive sections as thin or thick as your software demands, devoid of worrying about shedding or distorting beneficial tissue samples.
Megatome is usually a vibrating microtome made to part a broad variety of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With superior blade vibrating frequency and minimized blade deflection, Megatome enables substantial-throughput tissue sectioning with uniform area profile, along with small tissue damage and data decline.
Antibodies may possibly acquire weeks to diffuse via just a few millimeters of tissue, that has a steep labeling gradient from surface to core.
Megatome is made for precision: the blade vibrates at an increased frequency and bigger amplitude range than other microtomes, and incorporates a exceptional deflection Command mechanism.
SE uses a rotational electric area to disperse extremely electromobile molecules (which include antibodies or surfactant micelles) all over a porous sample without damaging electrically charged structures inside the tissue. This permits 2-4 day clearing of intact organs,
venture to make an aggregated Javadoc. Within this instance, javadocTask should be established to allJavadoc in order that the right documentation is developed just before making the docset.
Megatome is a novel microtome that enables for high-precision sectioning of a variety of tissue samples – megatomi.com from organoids, to arrays of animal organs, to intact human brain hemispheres – with minimal tissue problems and information decline.
eFLASH is really a immediate tissue labeling strategy that allows for uniform total-organ staining in 20 rounds of labeling.
The SmartSPIM light-weight sheet microscope supplies unparalleled resolution and acquisition speed all over intact tissue volumes, though its modular design and style allows you to update and customize specific components.
Docset development requires at bare minimum two options: the title of your docset and The placement of your Javadoc information to incorporate within the docset.
This can make a docset named Sample in the current directory. Docset generation can be custom-made with optional arguments:
SHIELD avoids the variability of hydrogel embedding and the knowledge reduction from PFA preservation, preserving specimens for numerous rounds of processing.
--displayName: Will established the name as proven in Dash. This is certainly helpful in the event you develop a docset with identify SampleProject but display identify Sample Venture in its place. This setting will default to the worth of --identify if omitted.
Our novel Defend tissue preservation procedure sorts intramolecular bonds using polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.